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美国ISS ChronosDFD数字频域荧光光谱仪

简介︰

New from ISS!

ChronosDFD 用于复式衰减荧光寿命测试小于1s

ChronosDFD通过Vinci软件实现全自动控制,操作环境友好,基于Windows系统的软件控制包。


产品概述和特点

ChronosDFD 主要特点:

·        灵活配置,可选光源(laser diodes, LEDs Ti:Sapphire laser)

·        体积小巧,光路短,且能到到最大灵敏度和光照效率。

·        毫秒至皮秒荧光寿命测试能力。

·        全自动化仪表组件:透明小容器,偏振器,快门,滤光轮,单色仪和搅拌器。

·        PC-控制集成恒温仪,滴定仪,停流设备和压力泵

·        可升级包含稳态测试

·        T-型和平行光束设计,已达到快速高精偏振测量

·         Vinci-Multidimensional 荧光光谱仪提供技术驱动

频域下获得数据的特点:

·        荧光寿命的计算取决于两个测量参数:相位角和调制

·        测量更快和更少操作

·        允许一步测量各向异性衰减(rotational correlation times)

·        更好的分辨较短时间寿命的影响

·        基于荧光寿命传感和实时采集的一种方法选择,由于在毫秒时间范围内高采集率。


技术参数


ChronosDFD配置

光源

  • 激光二极管 (nm): 370, 405, 436, 473, 635, 690, 780, 830

  • LEDs (nm): 280, 300, 335, 345, 460, 500, 520

  • 脉冲激光器: 超连续, Ti:Sapphire钛宝石激光器, 脉冲激光二极管

调焦&采集结构

平行光束设计以实现高精偏测量

偏光器

UV grade Glan-Thompson with L/A=2.0

检测器

  • PMT

  • hybrid PMT

  • MCP

  • APD

检测模式

光电子计数

波长范围

200 nm- 1700nm (探测器有关)

最大计数范围

可达1300counts/s (使用混合式探测器)

荧光寿命测量范围

10-12 s- 1 s-,
(软件控制可选)

操作系统

Windows 10

电源

通用电源输入: 110-240 V, 50/60 Hz, 400 VAC

尺寸

540 mm (L) x 425 mm (W) x 235 mm (H)

重量

25 kg

ChronosDFD示意图

http://www.iss.com/image/chronosDFD/diagram_chronosDFD.png


ChronosDFD测量举例

http://www.iss.com/image/chronosDFD/graph_fluorescein.gif

丙二醇中的荧光素

丙二醇中的荧光素频域各向异性衰减 (不同偏振相角和振幅比) ,使用 ChronosDFD 470-nm (氙灯)下测试, 发射线收集使用的是 OG530 高通滤波器 计算值r &thera; = 5.3 ns R0 = 0.40 τ = 4 ns, T = 27-28°C.

http://www.iss.com/image/chronosDFD/graph_bodipyfl.gif

BodipyFL氟硼二吡咯)水溶液

BodipyFL(氟硼二吡咯)水溶液频率响应 (相位和调制) 使用ChronosDFD 471-nm激光二极管发射线收集使用的是 520KV高通滤波器 数据的最佳拟合呈现指数衰减方式,衰减时间5.87 ns (x2 = 0.97).

ChronosDFD配件可选

详细信息请垂询010-82782023

  • 激光二极管光发射二极管(脉冲)

  • 白光激光器

  • 独立光源灯

  • 两透明容器样品室

  • 透明容器样品室

  • 透明容器样品室Peltier控制,样品室

  • 杜瓦瓶

  • HPCell™ 高压细胞系统

  • 全内置反射荧光(TIRF)细胞流通池

  • 可变角度正面样品室表面

  • 真空仓

  • 微孔板附件

  • UV Glan-Thompson 棱镜偏振器

  • 滴定器(1 2 )

  • Stopped-Flow 设备

  • 光纤光路

  • 计算机控制滤光轮

  • 微通道板

ChronosDFD应用领域及发表应用期刊

生物化学&分子生物学(细胞膜,核酸蛋白质)

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Cotranslational Protein Folding Within the Ribosome Tunnel Influences Trigger-factor Recruitment.
Lin, K.F., Sun, C.S., Huang, Y.C., Chan, S.I., Koubek, J., Wu, T.H., Huang, J.J.
Biophys J., 2012, 102(12), 2818-27.

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The N-terminus of TDP-43 Promotes Its Oligomerization and Enhances DNA Binding Affinity.
Chang, C.K., Wu, T.H., Wu, C.Y., Chiang, M.H., Toh, E.K., Hsu, Y.C., Lin, K.F., Liao, Y.H., Huang, T.H., Huang, J.J.
Biochem Biophys Res Commun., 2012, 425(2), 219-24.

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Efficient Isolation of Pseudomonas Aeruginosa Type III Secretion Translocators and Assembly of Heteromeric Transmembrane Pores in Model Membranes.
Romano, F.B., Rossi, K.C., Savva, C.G., Holzenburg, A., Clerico, E.M., Heuck, A.P.
Biochemistry., 2011, 50(33), 7117-31.

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Production of Ribosome-released Nascent Proteins With Optimal Physical Properties.
Ziehr, D.R., Ellis, J.P., Culviner, P.H., Cavagnero, S.
Anal Chem., 2010, 82(11), 4637-43.

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Excited-state Lifetime Studies of the Three Tryptophan Residues in the N-lobe of Human Serum Transferrin
James, N.G., Ross, J.A., Mason, A.B., Jameson, D.M.
Protein Science, 2010, 19, 99-110.

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Confined Dynamics of a Ribosome-bound Nascent Globin: Cone Angle Analysis of Fluorescence Depolarization Decays in the Presence of Two Local Motions.
Ellis, J.P., Culviner, P.H., Cavagnero, S.
Protein Sci., 2009, 18(10), 2003-15.

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Structural and Thermodynamic Characterization of T4 Lysozyme Mutants and the Contribution of Internal Cavities to Pressure Denaturation.
Ando, N., Barstow, B., Baase, W.A., Fields, A., Matthews, B.W., Gruner, S.M.
Biochemistry., 2008, 47(42), 11097-109.

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Chain Dynamics of Nascent Polypeptides Emerging From the Ribosome.
Ellis, J.P., Bakke, C.K., Kirchdoerfer, R.N., Jungbauer, L.M., Cavagnero, S.
ACS Chem Biol., 2008, 3(9), 555-66.

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Fructose-1,6-bisphosphate Acts Both as an Inducer and as a Structural Cofactor of the Central Glycolytic Genes Repressor (CggR).
Zorrilla, S., Chaix, D., Ortega, A., Alfonso, C., Doan, T., Margeat, E., Rivas, G., Aymerich, S., Declerck, N., Royer, C.A.
Biochemistry., 2007, 46(51), 14996-5008.

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Inducer-Modulated Cooperative Binding of the Tetrameric CggR Repressor to Operator DNA
Zorrilla, S., Doan, T., Alfonso, C., Margeat, E., Ortega, A., Rivas, G., Aymerich, S., Royer, C.A., Declerck, N.
Biophsyical J., 2007, 92(9), 3215-3227.

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Hydration of the Folding Transition State Ensemble of a Protein.
Brun, L., Isom, D.G., Velu, P., García-Moreno, B., Royer, C.A.
Biochemistry., 2006, 45(11), 3473-80.

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Time-resolved Fluorescence Anisotropy Studies Show Domain-specific Interactions of Calmodulin With IQ Target Sequences of Myosin V.
Bayley, P., Martin, S., Browne, P., Royer, C.
Eur Biophys J., 2003, 32(2), 122-7.

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Reorientational Dynamics of Enzymes Adsorbed on Quartz: A Temperature-Dependent Time-Resolved TIRF Anisotropy Study
Czeslik, C., Royer, C., Hazlett, T., Mantulin, W.
Biophys. J., 2003, 84, 2533-2541.

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Equilibrium Binding of Estrogen Receptor With DNA Using Fluorescence Anisotropy.
Ozers, M.S., Hill, J.J., Ervin, K., Wood, J.R., Nardulli, A.M., Royer, C.A., Gorski, J.
J Biol Chem., 1997, 272(48), 30405-11.

环境研究

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Real-Time Determination of Picomolar Free Cu(II) in Seawater Using a Fluorescence-Based Fiber Optic Biosensor
Zeng, H.-H., Thompson, R. B., Maliwal, B. P., Fones, G. R., Moffett, J. W., Fierke, C. A.
Anal. Chem., 2003, 75(24), 6807-6812.

化工制药

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Characterization of Fluorinated Catansomes: A Promising Vector in Drug-delivery.
Rosholm, K.R., Arouri, A., Hansen, P.L., González-Pérez, A., Mouritsen, O.G.
Langmuir., 2012, 28(5), 2773-81.

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Indocyanine Green-Loaded Biodegradable Nanoparticles: Preparation, Physicochemical Characterization and in Vitro Release
Saxena, V., Sadoqi, M., Shao., J.
Int. J. Pharm., 2004, 278(2), 293-301.

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Enhanced Photo-Stability, Thermal-Stability and Aqueous-Stability of Indocyanine Green in Polymeric Nanoparticulate Systems
Saxena, V., Sadoqi, M., Shao., J.
J. Photochem. Photobiol., 2004, 74(1) 29-38.

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Degradation Kinetics of Indocyanine Green in Aqueous Solution
Saxena, V., Sadoqi, M., Shao., J.
J. Pharm Sci.,2003, 92(10), 2090-2097.

物理化学

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Developing Red-emissive Ruthenium(II) Complex-based Luminescent Probes for Cellular Imaging.
Zhang, R., Ye, Z., Yin, Y., Wang, G., Jin, D., Yuan, J., Piper, J.A.
Bioconjug Chem., 2012, 23(4), 725-33.

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Noncovalent Assembly of a Metalloporphyrin and an Iron Hydrogenase Active-site Model: Photo-induced Electron Transfer and Hydrogen Generation.
Li, X., Wang, M., Zhang, S., Pan, J., Na, Y., Liu, J., Akermark, B., Sun, L.
J Phys Chem B., 2008, 112(27), 8198-202.

传感器

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Water Soluble Indodicarbocyanine Dyes Based on 2,3-dimethyl-3-(4-sulfobutyl)-3H-indole-5-sulfonic Acid
Markova, L.I., Fedyunyayeva, I.A., Povrozin, Y.A., Semenova, O.M., Khabuseva, S.U., Terpetschnig, E.A., Patsenker, L.D.
Dyes and Pigments, 2013, 96(2), 535-46.

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Seta-633 - a NIR Fluorescence Lifetime Label For Low-Molecular-Weight Analytes
Povrozin, Y.A., Kolosova, O.S., Obukhova, O.M., Tatarets, A.L., Sidorov, V.I., Terpetschnig, E.A., Patsenker, L.D.
Bioconjug Chem., 2009, 20(9), 1807-12.

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Near-Infrared, Dual-Ratiometric Fluorescent Label for Measurement of pH
Povrozin, Y.A., Markova, L.I., Tatarets, A.L., Sidorov, V.I., Terpetschnig, E.A., Patsenker, L.D.
Anal Biochem., 2009, 390(2), 136-40.

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Synthesis of Water-Soluble, Ring-Substituted Squaraine Dyes and Their Evaluation as Fluorescent Probes and Labels
Tatarets, A.L., Fedyunyayeva, I.A., Dyubko, T.S., Povrozin, Y.A., Doroshenko, A.O., Terpetschnig, E.A., Patsenker, L.D.
Anal Chim Acta., 2006, 570(2), 214-23.

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Fatty Acid Sensor for Low-Cost Lifetime-Assisted Ratiometric Sensing Using a Fluorescent Fatty Acid Binding Protein
Bartolome, A., Bardliving, C., Rao G., Tolosa L.
Analytical Biochemistry, 2005, 34(1), 133-139.

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Dual-Labeled Glucose Binding Protein for Ratiometric Measurements of Glucose
Ge, X., Tolosa, L., Rao, G.
Analytical Chemistry, 2004, 76(5), 1403-10.

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Reagentless Optical Sensing of Glutamine Using a Dual-Emitting Glutamine-Binding Protein
Tolosa, L., Ge, X., Rao, G.
Analytical Biochemistry, 2003, 314(2), 199-205.


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